A PREPARAÇÃO NERVO FRÊNICO-DIAFRAGMA (CAMUNDONGOS/RATOS) E A TÉCNICA MIOGRÁFICA COMO FERRAMENTA FARMACOLÓGICA/ The phrenic nerve-diaphragm preparation (mice/rats) and the miographic technique as a pharmacological tool

Jocimar de Souza; Yoko Oshima-Franco; Nobel Penteado de Freitas

doi:10.26694/jibi.v5i2.10494

A PREPARAÇÃO NERVO FRÊNICO-DIAFRAGMA (CAMUNDONGOS/RATOS) E A TÉCNICA MIOGRÁFICA COMO FERRAMENTA FARMACOLÓGICA/ The phrenic nerve-diaphragm preparation (mice/rats) and the miographic technique as a pharmacological tool

Jocimar de Souza, Yoko Oshima-Franco, Nobel Penteado de Freitas

Resumo

A junção neuromuscular (JNM) é formada por sinapses entre um nervo motor e um músculo esquelético. Ensaios farmacológicos realizados na JNM são de extrema importância, pois permitem testar, validar e estudar a farmacodinâmica de diversas substâncias. Uma preparação neuromuscular muito utilizada para este propósito é a preparação ex vivo Nervo Frênico-Diafragma (NFD). O objetivo deste trabalho foi pesquisar sobre o uso da preparação NFD de camundongos/ratos através de técnica miográfica. A metodologia empregada na pesquisa foi realizada utilizando-se “rats or mouse phrenic nerve-diaphragm preparation” como descritores, nas bases de dados PubMed, Google Acadêmico, SciElo e Periódicos CAPES. Os critérios de inclusão foram artigos que utilizaram ambos, preparação NFD e técnica miográfica, no período de 1946 a 2019. A JNM representa anatomicamente a sinapse nervo-músculo, simples de ser isolada e fácil de ser estudada. Há diversos protocolos farmacológicos (pré-, pós- e na fenda sinaptica) utilizando-se essas preparações através da técnica miográfica, com resultados assertivos sobre a farmacodinâmica de determinadas substâncias. Feito o levantamento, a base de dados em que se obteve mais publicações foi o Google Acadêmico, com uma média de 29 artigos por ano (70% das publicações), seguida do Periódico CAPES com 11 artigos por ano em média (25,4% das publicações), PubMed com média de dois artigos por ano (4,2% das publicações) e, enfim, SciElo com média de um artigo por ano (0,4% das publicações). Em conclusão, o uso da preparação NFD de ratos/camundongos no período levantado mostra que, mesmo com a preocupação da Comissão de Ética para o Uso de Animais (CEUA), as pesquisas com animais foram recorrentes e de grande importância. Este robusto modelo experimental tem permitido abrir perspectivas para novas perguntas na área da ciência, podendo ser complementado com outros ensaios como bioquímicos, histológicos e imunohistoquímicos

Palavras-chave

Junção Neuromuscular; Preparação Nervo-Músculo; Camundongos; Bases de dados; Sinapses

Texto completo:

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DOI: https://doi.org/10.26694/jibi.v5i2.10494