ANTIMICROBIAL AND SYNERGISTIC ACTIVITY OF ESSENTIAL OILS FACING ISOLATED BACTERIA FROM SURGICAL STAFF’S
Resumo
Objective:to evaluate antimicrobial and synergistic activity of essential oils (Citrus aurantifolia, Citrus sinensis, Mentha viridisand Thymus vulgaris) against isolated bacteria from surgical staff’s hands and bacterial strains. Method:the antimicrobial activity of oils was analyzed by microdilution method to determine the Minimum Inhibitory Concentration. Moreover, the combinatory effect of the oil that presented greater effectiveness using gentamycin sulphate through the Fractional Inhibitory Concentration index was tested. The study was performed over the period 2016-2018. Results:T. vulgaris was effective against Staphylococcus saprophyticus with a concentration of 0.0008 µL.mL-1and against the ATCC standard strains of Staphylococcus aureus, Salmonella choleraesuis,Staphylococcus epidermidis and Proteus vulgarisand the bacteria S. aureus, S. epidermidisand Bacillus sp. with MIC ≥ 0.10 µL.mL-1. This oil association with gentamycin sulphate showed synergistic activity againstS. epidermidis. Conclusion:T. vulgarisessential oil showed expressive antimicrobial activity against ATCC and isolated clinic microorganisms, suggesting broad spectrum of activity.
Referências
Brasil. Ministério da Saúde. Portaria n.º 2616, 12 de maio de 1998. Dispõe sobre a Proposta de regulamentação técnica para o controle de ISC. Diário Oficial da União. 1998 maio 13; Seção 1:182-184. Available from: http://bvsms.saude.gov.br/bvs/saudelegis/gm/1998/prt2616_12_05_1998.html
Marras MA, Lapena SAB. Atuação da equipe multidisciplinar no combate a infecções hospitalares. J Health Sci Inst. [Internet]. 2015 Jan-Mar [cited 2019 Jan 30];33(1):37–44. Available from: https://www.unip.br/presencial/comunicacao/publicacoes/ics/edicoes/2015/01_jan-mar/V33_n1_2015_p37a44.pdf
World Health Organization. The Evolving Threat of Anti-microbial Resistance: Options for Action. Switzerland: WHO; 2012. Available from: http://whqlibdoc.who.int/publications/2012/9789241503181_eng.pdf
Centers for Disease Control and Prevention. Antibiotic Resistance Threats in the United States. United States of America: CDC; 2013. Available from: http://www.cdc.gov/drugresistance/threat-report-2013/pdf/ar-threats-2013-508
Hay SI, Rao PC, Dolecek C, Day NPJ, Stergachis A, Lopez AD, et al. Measuring and mapping the global burden of antimicrobial resistance. BMC Med [Internet]. 2018 Jun [cited 2019 Jan 30];16(1):78-80. Available from: https://doi.org/10.1186/s12916-018-1073-z
Carvalho RLR, Campos CC, Franco LMC, Rocha AM, Ercole FF. Incidência e fatores de risco para infecção de sítio cirúrgico em cirurgias gerais. Rev Lat Am Enfermagem [Internet]. 2017 Dec [cited 2019 Jan 30];25:e2848. Available from: http://doi.org/10.1590/1518-8345.1502.2848
Sievert DM, Ricks P, Edwards JR, Schneider A, Patel J, Srinivasan A, et al. Antimicrobial-Resistant Pathogens Associated with Healthcare-Associated Infections: Summary of Data Reported to the National Healthcare Safety Network at the Centers for Disease Control and Prevention, 2009–2010. Infect Control Hosp Epidemiol [Internet]. 2013 Jan [cited 2019 Jan 30];34(1):1-14. Available from: http://www.jstor.org/stable/10.1086/668770
World Health Organization. Global Guidelines for the Prevention of Surgical Site Infection. Switzerland: WHO; 2016. Available from: http://apps.who.int/iris/bitstream/handle/10665/250680/9789241549882-eng.pdf;jsessionid=159DA0AC4F39D19596320740FA1529AA?sequence=1
Oliveira AC, Gama CS. Antissepsia cirúrgica e utilização de luvas cirúrgicas como potenciais fatores de risco para contaminação transoperatória. Escola Anna Nery Revista de Enfermagem [Internet]. 2016 Apr-Jun [cited 2019 Jan 30];20(2):370-377. Available from: http://doi.org/10.5935/1414-8145.20160051
Bakkali F, Averbeck S, Averbeck D, Idaomar M. Biological effects of essential oils – A review. Food Chem Toxicol [Internet]. 2008 Feb [cited 2019 Jan 30];46(2):446–475. Available from: http://doi.org/10.1016/j.fct.2007.09.106
Duarte MCT. Atividade Antimicrobiana de Plantas Medicinais e Aromáticas Utilizadas no Brasil. Revista MultiCiência [Internet]. 2006 Oct [cited 2019 Jan 30];7:01-16. Available from: https://www.multiciencia.unicamp.br/artigos_07/a_05_7.pdf
Ferreira LE, Benincasa BI, Fachin AL, Contini SSHT, França SC, Chagas ACS, et al. Essential oils of Citrus aurantifolia, Anthemis nobile and Lavandula officinalis: in vitro anthelmintic activities against Haemonchus contortus. Parasit & Vectors [Internet]. 2018 Apr [cited 2019 Jan 30];11(269):2-3. Available from: https://doi.org/10.1186/s13071-018-2849-x
Khodavaisy S, Nabili M, Davari B, Vahedi M. Evaluation of bacterial and fungal contamination in the health care workers’ hands and rings in the intensive care unit. J Prev Med Hyg [Internet]. 2011 Dec [cited 2019 Jan 30];52(4):215-218. Available from: https://pdfs.semanticscholar.org/4d0f/113eb52fb0c4b24f777e34de7c3f7878d409.pdf
Murray PR, Baron EJ, Pfaller MA, Jorgensen JH, Yolken RH. Manual of clinical microbiology. 6th ed. Washington, DC: ASM Press; 1995.
Clinical and Laboratory Standards Institute. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically. 11th ed. document M07-A10; 2018.
Pappalardo MCSM. Parâmetros farmacocinéticos e farmacodinâmicos da anfotericina B e fluconazol e sua contribuição no estudo da correlação clínico-laboratorial da criptococose de sistema nervoso central associada à AIDS [tese de doutorado]. São Paulo: Programa de Pós-Graduação em Ciências da Coordenadoria de Controle de Doenças da Secretaria de Estado da Saúde de São Paulo; 2009.
Ferreira LE, Benincasa BI, Fachin AL, França SC, Contini SSHT, Chagas ACS, et al. Thymus vulgaris L. essential oil and its main component thymol: Anthelmintic effects against Haemonchus contortus from sheep. Vet Parasitol [Internet]. 2016 Sep [cited 2019 Jan 30];228:70-76. Available from: http://doi.org/10.1016/j.vetpar.2016.08.011
Zhu J, Zeng X, Yanma, Liu T, Qian K, Han Y, et al. Adult repellency and larvicidal activity of five plant essential oils against mosquitoes. J Am Mosq Control Assoc [Internet]. 2006 Sep [cited 2019 Jan 30];22(3):515-522. Available from: http://doi.org/10.2987/8756-971X(2006)22[515:ARALAO]2.0.CO;2
Abu-Darwish MS, Al-Ramamneh EA, Kyslychenko VS, Karpiuk UV. The antimicrobial activity of essential oils and extracts of some. Pak J Pharm Sci [Internet]. 2012 Jan [cited 2019 Jan 30];25(1):239-246. Available from: https://www.ncbi.nlm.nih.gov/pubmed/22186336
Segvić-Klarić M, Kosalec I, Mastelić J, Piecková E, Pepeljnak S. Antifungal activity of thyme (Thymus vulgaris L.) essential oil and thymol against moulds from damp dwellings. Lett Appl Microbiol [Internet]. 2006 Sep [cited 2019 Jan 30];44(1):36-42. Available from: https://doi.org/10.1111/j.1472-765X.2006.02032.x
Qadir MA, Shahzadi SK, Bashir A, Munir A, Shahzad S. Evaluation of Phenolic Compounds and Antioxidant and Antimicrobial Activities of Some Common Herbs. Int J Anal Chem [Internet]. 2017 Feb [cited 2019 Jan 30];2017:1-6. Available from: https://doi.org/10.1155/2017/3475738
Adwan G, Abu-Shanab B, Adwan K, Abu-Shanab F. Antibacterial effects of nutraceutical plants growing in Palestine on Pseudomonas aeruginosa. Turk J Biol [Internet]. 2006 Jan [cited 2019 Jan 30];30(4):239–242. Available from: https://www.researchgate.net/publication/256979569_Antibacterial_Effects_of_Nutraceutical_Plants_Growing_In_Palestine_on_Pseudomonas_Aeruginosa
Al-Bayati FA. Synergistic antibacterial activity between Thymus vulgaris and Pimpinella anisum essential oils and methanol extracts. J Ethnopharmacol [Internet]. 2008 Mar [cited 2019 Jan 30];116(3):403-406. Available from: https://doi.org/10.1016/j.jep.2007.12.003
Tohidpour A, Sattari M, Omidbaigi R, Yadegar A, Nazemi J. Antibacterial effect of essential oils from two medicinal plants against Methicillin-resistant Staphylococcus aureus (MRSA). Phytomedicine [Internet]. 2010 Feb [cited 2019 Jan 30];17(2):142–145. Available from: https://doi.org/10.1016/j.phymed.2009.05.007
Bourgou S, Rahali FZ, Ourghemmi I, Saïdani Tounsi M. Changes of peel essential oil composition of four Tunisian citrus during fruit maturation. ScientificWorldJournal [Internet]. 2012 [cited 2019 Jan 30]. Available from: http://doi.org/10.1100/2012/528593
Silva LF. Mentha viridis (L). L. e Mentha Pulegium L.: caracterização química, atividades antibacteriana, antioxidante, fosfolipásica, hemolítica e genotóxica dos óleos essenciais [tese de mestrado]. Lavras: Universidade Federal de Lavras, DQI - Departamento de Química; 2014.
DOI: https://doi.org/10.26694/repis.v5i0.8970
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