Antimicrobial activity and cytotoxic assessment of gallic and ellagic acids

Michel Mualem de Moraes Alves; Lucas Moreira Brito; Adriana Cunha Souza; Thaynara Parente de Carvalho; Felipe José Costa Viana; Felipe Araújo de Alcântara Oliveira; Humberto Medeiros Barreto; Jéssica Sara de Sousa Macêdo Oliveira; Mariana Helena Chaves; Daniel Dias Rufino Arcanjo; Fernando Aécio de Amorim Carvalho

doi:10.26694/jibi.v3i1.6626

Antimicrobial activity and cytotoxic assessment of gallic and ellagic acids

Michel Mualem de Moraes Alves, Lucas Moreira Brito, Adriana Cunha Souza, Thaynara Parente de Carvalho, Felipe José Costa Viana, Felipe Araújo de Alcântara Oliveira, Humberto Medeiros Barreto, Jéssica Sara de Sousa Macêdo Oliveira, Mariana Helena Chaves, Daniel Dias Rufino Arcanjo, Fernando Aécio de Amorim Carvalho

Resumo

Os objetivos deste estudo foram investigar a atividade antimicrobiana do ácido gálico (AG) e ácido elágico (AE) contra estirpes de bactérias, Candida albicans e Leishmania amazonensis, bem como avaliar sua citotoxicidade em macrófagos peritoneais murinos. As concentrações mínimas de inibição (CMI) de ácidos gálico e elágico foram determinadas pelo ensaio de microdiluição. As CMIs da norfloxacina contra uma estirpe de Staphylococcus aureus com sobre-expressão de NorA foram determinadas na ausência ou presença de cada composto em concentrações sub-inibitórias, a fim de verificar a capacidade destes compostos como potenciais inibidores da bomba de efluxo. O ácido gálico foi inativo contra todas as cepas testadas, enquanto isso o ácido elágico mostrou atividade contra S. aureus e C. albicans. Por outro lado, ambos os compostos não conseguiram modular a resistência à fluoroquinolona, indicando que não são inibidores de NorA. Além disso, eles foram ativos contra L. amazonensis, com valores de IC50 de 10,94 e 3,64 μg ∙ mL-1 para AG e AE, respectivamente. Eles também mostraram citotoxicidade em macrófagos peritoneais murinos com valores de CC50 de 126,5 e 23,811 μg ∙ mL-1 para AG e AE, respectivamente. Curiosamente, ambos os compostos mostraram ser mais seletivos para parasitas que para macrófagos. Estes resultados demonstraram que os ácidos gálico e elágico representam uma alternativa potencial para a terapia de infecções por estafilococos, micoses e leishmaniose.

Palavras-chave

Candida, Leishmania, Macrophage, Staphylococcus, Tannins

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DOI: https://doi.org/10.26694/jibi.v3i1.6626